Nonetheless, non-conventional system with our current MHMPD prototype could not advocate a better outcome. For follicle density (primordial to secondary stage), conventional culture system also yielded higher result than MHMPD. Growth rates from primordial to primary follicles were 2.2% and 1.9% in control and MHPD groups, respectively. Results: Our preliminary data demonstrated that mean (SD) percentage of normal follicle morphology (primordial to secondary stage) in fresh tissues and control were 78.1 ± 1.9 and 73.4 ± 3.9 whereas 69.2 ± 4.7 in MHMPD group. Ovarian tissues were cultured for four days and histologically evaluated for follicle morphology, follicle development growth rate and follicle density. Ovarian cortical pieces (0.2 cm x 0.2 cm x 0.1 cm width x length x thickness) from each animal were divided into two groups I) conventional culture system (control) and II) non-conventional system with MHMPD (fabricated from standard soft lithography technique using Polydimethylsiloxane connected with modified media tank and calculated for legitimated flow rate 0 – 10 hours: 0.0003 mL/min and 11 – 20 hours: 0.00083 mL/min and constant flow rate after 20 hours). Methods: Ovarian tissues were retrieved from four wild-type mice aged 2-3 months under approval by Institutional Animal Care and Use Committee, Faculty of Medicine, Chulalongkorn University. This study aimed to compare efficacy of conventional (static culture system) and non-conventional (modified hydrostatic microfluidic pumpless device MHMPD) systems on IVA using mouse model. However, this culture system propagates through missing of natural blood circulation mimicking leading to cell to cell interaction receding.
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Fundamental in vitro tissue culture protocol, static culture system, with sequential culture media changed every day two consecutive days has been generally performed. Introduction: In vitro follicle activation (IVA) theoretically involves with ovarian cortical culture for period of time. Thuwanut, Paweena 1 Pimpin, Alongkorn 1 Sereepapong, Wisan 2 Pruksananonda, Kamthorn 2 Suebthawinkul, Chanakarn 2 Tuntiviriyapun, Punkavee 2 Thaweepolcharoen, Charoen 2 Sirayapiwat, Porntip 3
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A MODIFIED HYDORSTATIC MICROFLUIDIC PUMPLESS DEVICE: A PRELIMINARY STUDY AS AN ALTERNATIVE APPROCH FOR IN VITRO FOLLICLE ACTIVATION IN MOUSE MODEL